Free ยท Private ยท No Account Required

Professional
DNA Analysis
in Your Browser

The most powerful client-side bioinformatics toolkit. Primer design with SantaLucia thermodynamics, restriction mapping, codon optimization, ORF finding, and 10+ more tools. All running locally, all free.

Launch Analyzer Explore Features โ†“
10+
Analysis Tools
100%
Client-Side
0
Sequences Uploaded
โœ“ Tm = 62.4ยฐC ๐Ÿ”ฌ 3 ORFs Found โšก Instant Results
sequence_analysis.dna
Length
240 bp
GC Content
58.3%
Tm (avg)
62.4ยฐC
ORFs Found
3
GC Content58.3%

Everything You Need for
Sequence Analysis

A complete bioinformatics suite built for researchers, students, and professionals. No installation, no subscription.

๐Ÿงช
Primer Design
SantaLucia nearest-neighbor thermodynamics for accurate Tm. Hairpin/dimer ฮ”G, 3โ€ฒ stability, GC clamp, poly-run detection, and heterodimer checking.
SantaLucia NNHairpin ฮ”GHeterodimer
โœ‚๏ธ
Restriction Enzyme Mapping
Visual linear restriction map with canvas-based ruler, backbone, staggered cut markers, per-enzyme lanes, and an interactive legend.
Visual Map50+ EnzymesCanvas
๐Ÿ”„
Codon Optimization
CAI-weighted RSCU engine with rare codon flagging, per-codon frequency table, and CAI score display for multiple host organisms.
CAI ScoreRSCURare Codons
๐Ÿ”
ORF Finder
Detect open reading frames across all 6 reading frames. Translates to amino acid sequences with codon-by-codon highlighting.
6 FramesTranslationHighlighting
๐Ÿ๏ธ
CpG Island Detection
Identifies CpG islands using observed/expected ratio and GC content thresholds. Visualized with a color-coded map overlay.
Obs/Exp RatioVisual Map
๐ŸŒ€
RNA Secondary Structure
Predicts RNA hairpin structures using free energy minimization. Displays stem-loop diagrams with ฮ”G values.
Hairpinฮ”GStem-Loop
๐Ÿ“Š
GC Content Analysis
Window-based GC% sliding analysis with interactive chart. Highlights AT-rich and GC-rich regions across the full sequence.
Sliding WindowChart
๐Ÿ”
Repeat Finder
Detects tandem repeats, inverted repeats, and direct repeats. Annotates positions and repeat unit lengths across the sequence.
TandemInvertedDirect
๐ŸŽฏ
Kozak Scorer & AA Properties
Scores Kozak consensus strength for translation initiation. Amino acid property analysis including hydrophobicity, charge, and molecular weight.
KozakHydrophobicityMW

Up and Running in Seconds

No installation, no sign-up. Just paste your sequence and get results instantly.

1
Paste Your Sequence
Paste any DNA sequence in FASTA or raw format. Supports sequences up to hundreds of kilobases.
2
Choose Your Analysis
Select from 10+ analysis modules: primer design, restriction mapping, codon optimization, and more.
3
Get Instant Results
Results render immediately in your browser. No server round-trips, no waiting, no data leaving your device.
4
Export & Share
Copy results, export tables, and share findings directly from the app with one click.

Your Sequences Never Leave
Your Browser

๐Ÿ”’
Sequences Never Uploaded
Every computation runs locally in JavaScript. Your sequences are never sent to any server, not even ours.
๐Ÿšซ
No Account Required
No sign-up, no email, no paywall. Open the app and start analyzing immediately.
๐Ÿ“ด
Works Offline
Once loaded, the analyzer works without an internet connection. Works great in secure lab environments.
โšก
Instant Performance
No server latency. Results are computed in milliseconds directly on your hardware.
๐Ÿ“ˆ
Analytics, Stated Plainly
We use Google Analytics to count visits and see which tools get used. It records standard web analytics data and sets cookies. It never sees the sequences you paste.
๐Ÿ›ก๏ธ
100% Client-Side Processing
Your sequences stay on your machine.
Analyzed locally, never uploaded.
FAQ

Frequently Asked Questions

What researchers ask most before pasting their first sequence.

Is PrimerGenius free to use?
Yes. Every tool is free with no account, no sign-up and no usage limits. There is no paid tier.
Are my DNA sequences uploaded to a server?
No. All analysis runs in JavaScript inside your own browser. Your sequences never leave your device, so the tool is safe to use with unpublished or confidential data. Page-visit analytics are collected separately and never include your sequence.
How does PrimerGenius calculate melting temperature?
Primer Tm is calculated with SantaLucia nearest-neighbor thermodynamics, which accounts for stacking energies between adjacent bases rather than using a simple GC percentage formula.
What sequence formats are supported?
Plain raw sequence and FASTA are both accepted. Whitespace, line breaks and numbering are stripped automatically, and sequences of several hundred kilobases are supported.
Can I use PrimerGenius offline?
Yes. Once the page has loaded, the analyzer keeps working without an internet connection, which makes it usable on air-gapped lab machines.
Do you use cookies or analytics?
Yes. The site runs Google Analytics to measure page visits and which tools are used, and that sets cookies in your browser. It collects standard web analytics data such as pages viewed, referrer, approximate region and device type. It has no access to the sequences you paste, because those are never sent anywhere.
Which analyses are included?
Primer design, restriction enzyme mapping, codon optimization, ORF finding across six frames, CpG island detection, RNA secondary structure, GC content analysis, repeat finding, Kozak scoring and amino acid property analysis.

Ready to Analyze
Your Sequence?

Used by researchers worldwide. The most capable free DNA analysis tool you can run in a browser.

Launch Analyzer (Free)